Journal: Biomarker Insights

Article Title: Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD

doi: 10.4137/BMI.S28209

Figure Lengend Snippet: Auto-Ab identification methods used in this study. ( A ) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. ( b ) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). ( C ) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. ( D ) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.

Article Snippet: Immune response biomarker profiling was performed with ProtoArray Human Protein Micro-array v5.0 (Invitrogen) for detection of auto-Abs in pooled sera from 15 patients with collagen disease either with AoDILD or in the stable state, according to the manufacturer’s protocol.

Techniques: Immunofluorescence, Amplified Luminescent Proximity Homogenous Assay, Enzyme-linked Immunosorbent Assay

Journal: Biomarker Insights

Article Title: Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD

doi: 10.4137/BMI.S28209

Figure Lengend Snippet: Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with ( A ) ProtoArray and ( b ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between ( C ) ProtoArray and ( D ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).

Article Snippet: Immune response biomarker profiling was performed with ProtoArray Human Protein Micro-array v5.0 (Invitrogen) for detection of auto-Abs in pooled sera from 15 patients with collagen disease either with AoDILD or in the stable state, according to the manufacturer’s protocol.

Techniques: Amplified Luminescent Proximity Homogenous Assay

Journal: Neuron

Article Title: High-resolution CBV-fMRI allows mapping of laminar activity and connectivity of cortical input and output in human M1

doi: 10.1016/j.neuron.2017.11.005

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins Critical Commercial Assays Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains healthy human volunteers this paper ClinicalTrials.gov identifier: {"type":"clinical-trial","attrs":{"text":"NCT00001360","term_id":"NCT00001360"}} NCT00001360 Post mortem MRI data ( Stüber et al., 2014 ) Fig. S1 ; https://doi.org/10.1016/j.neuroimage.2014.02.026 Oligonucleotides Recombinant DNA Software and Algorithms SIEMENS VB17A- UHF image reconstruction SIEMENS Healthineers IcePAT WIP 571 FSL Software Library v5.0 FMRIB, Oxford https://fsl.fmrib.ox.ac.uk/fsl/fslwiki Analysis of Functional NeuroImages (AFNI) AFNI_2011_12_21_2014 and AFNI_17.2.05 NIMH https://afni.nimh.nih.gov/ Statistical Parametric Mapping v12 Wellcome Trust Centre for Neuroimaging, UCL http://www.fil.ion.ucl.ac.uk/spm/ Stability weighted RF-coil combination (STARC) this paper https://github.com/djangraw/STARC-OptimalCoilCombo Other Open in a separate window KEY RESOURCE TABLE

Techniques: Virus, Recombinant, Software, Functional Assay

Journal: Frontiers in Genetics

Article Title: Current Status of Next-Generation Sequencing Approaches for Candidate Gene Discovery in Familial Parkinson´s Disease

doi: 10.3389/fgene.2022.781816

Figure Lengend Snippet: List of published studies that identified novel Parkinson’s disease loci using next-generation sequencing approaches.

Article Snippet: Quadri et al. (2018) , LRP10 (Low-density lipoprotein receptor - related protein 10) , Italian family , Genome-wide SNP array genotyping and linkage analysis in ten affected , WES on index PD patient (HiSeq 2000, Illumina) , Burrows-Wheeler Aligner (BWA-MEM version 0.5.9 (read alignment to human References genome - UCSC hg19) , Genome-Analysis-Tool-Kit (GATk) v3 (variant calling) , Cartagenia Bench Lab NGS v·5·0·1 (variant filtering) , Variants were excluded if , Homozygous.

Techniques: Next-Generation Sequencing, Sequencing, Variant Assay, In Silico, Software, Mutagenesis, Genome Wide, Clone Assay, Expressing, Functional Assay